CALYPSO™ CG-MALS en Goleta, California, USA

The CALYPSO software uses CG-MALS data to calculate the affinity and stoichiometry of specific, reversible complexes formed in solution, such as antibody-antigen interactions and protein dimerization. The software enables quick data fitting to simple models of dimer formation, 1:n stoichiometries, and infinite self-association. Alternatively, the highly versatile analysis tools allow you to choose and design more complex binding schemes, including simultaneous self- and hetero-association, cooperativity, and meta-complex formation.
The second virial coefficient (A2) is used to quantify nonspecific attractive and repulsive interactions for proteins, polymers, and colloids. Batch light scattering data collected with the CALYPSO software can be analyzed using a Zimm formalism, Debye formalism, or effective hard sphere approximation. Higher virial coefficients may be determined as well.
Some protein association or dissociation reactions can take seconds or minutes to reach completion.